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. 2018 Mar 27;8:5271. doi: 10.1038/s41598-018-23593-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transient LV productions by transfecting HEK293T cells. (a) Transient production titer comparison of LV pseudotyped with VSV-G or amphotropic envelope, using the wild type or the mutated T26S protease. (b) Transient LV production titer comparison of Gag-Pro(T26S)-Pol expression cassettes. (c) Transient LV production titer comparison of Rev expression cassettes. (d) Transient LV production titer comparison of vector genome expression cassettes. The (+) indicates presence and the (−) indicates absence of the respective plasmid in the transfection mix. Data shown represents the average ± standard deviation from 3 independent experiments. *(p < 0.05) and **(p < 0.005) given by one tailed unpaired t-test.