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. 2018 Mar 27;8:5271. doi: 10.1038/s41598-018-23593-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Influence of sodium butyrate in LentiPro26-A59 viral production. (a) Viable cell concentration of LentiPro26-A59 cell culture, 24 hours after medium exchange by fresh medium with increasing sodium butyrate concentrations. (b) Volumetric titers of stable LV productions performed using increasing sodium butyrate concentrations. (c) Viable cell concentration over a prolonged cell culture period in the presence or absence of sodium butyrate. The black arrow represents the time point when the medium started to be exchanged by fresh medium with 5 mM of sodium butyrate. (d) Volumetric titers obtained in 24 hours production periods over a prolonged cell culture period in the presence or absence of sodium butyrate. All data shown represents the average ± standard deviation from 2 independent experiments.