Table 1.

Expression of SGLT2 and SGLT1 mRNA in human endothelial cells. Expression of SGLT2, SGLT1 relative to TBP was assessed by qPCR in cDNA prepared from three independent cultures of HUVECs, HAECs and HEK-293 cells, using cDNA from human kidney cortex as a positive control. Data shown are the mean ± SEM Ct values and 2^−ΔΔCt in each case, from three independent samples. ND = Not detected.

Sample	Ct (TBP)	Ct (SGLT1)	SGLT1 2−ΔΔCt	Ct (SGLT2)	SGLT2 2−ΔΔCt
Human kidney cortex	25.00	22.68	1	17.73	1
HAEC	23.67 ± 0.39	34.65 ± 0.23	1.04 × 10−4 ± 0.22 × 10−4	ND	ND
HUVEC	23.53 ± 0.12	34.24 ± 0.29	1.26 × 10−4 ± 0.28 × 10−4	ND	ND
HEK-293	22.10 ± 0.16	30.89 ± 0.45	4.61 × 10−4 ± 0.94 × 10−4	ND	ND