. 2018 Mar 27;8:5231. doi: 10.1038/s41598-018-23485-1

Table 3.

Primers used to produce recombinant forms of the ligands.

No.	Protein/(Gene)	Sequence used to design primers	Primer	Sequence (5′-3′)	Amplicon length (bp)
1	Adhesion lipoprotein(Spr0906)	AE007317.1*: nt891426 to nt892361	spr0906- sense	TATAGATCTGGTGACTTGAATGATGTTCGG	717
1	Adhesion lipoprotein(Spr0906)	AE007317.1*: nt891426 to nt892361	spr0906- antisense	TTTGTCGACGGTCTTGTCATTTTGTGGGTC	717
2	Hypothetical protein(spr1730)	AE007317.1*: ntc1708366 to nt1707692	spr1730- sense	TATGGATCCGGGAATTTTGGAATTCCTTCT	597
2	Hypothetical protein(spr1730)	AE007317.1*: ntc1708366 to nt1707692	spr1730- antisense	TTTGTCGACTTCCAACTTCCCAAAAGCCAC	597
3	LPXTG-motif cell wall anchor protein(spr0440)	AE007317.1*: nt441113 to nt446092	spr0440- sense	TATGGATCCGCAGGTCACCGTAACGGGGTT	2238
3	LPXTG-motif cell wall anchor protein(spr0440)	AE007317.1*: nt441113 to nt446092	spr0440- antisense	TTTGTCGACTGCTAGGTCTCCTCCAACTTCGCT	2238
4	Hypothetical protein (spr0777)	AE007317.1*: nt771852 to nt772961	spr0777- sense	TATGGATCCGGTGTTGTCTATCTCTTACCTATTTTG	216
4	Hypothetical protein (spr0777)	AE007317.1*: nt771852 to nt772961	spr0777- antisense	TTTGTCGACCCCCACACTATTTGATACGCT	216
5	Pneumococcal histidine triad protein A (Spr1061)	AE007317.1*: ntc1059416 to nt1056930	spr1061- sense	TATGGATCCGAGTTGGGACTGTATCAAGCT	2367
5	Pneumococcal histidine triad protein A (Spr1061)	AE007317.1*: ntc1059416 to nt1056930	spr1061- antisense	TTTGTCGACACTTACAGATGAAGGATTACTTCC	2367

*Shows Genbank accession number followed by nucleotide positions spanning the gene. Restriction sites are depicted with underlined nucleotides. AGATCT – BglII, GGATCC – BamHI and GTCGAC – SalI.