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. 2018 Mar 27;8:5272. doi: 10.1038/s41598-018-23622-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Kanamycin sensitive (Kan^S) clones appearance after DSB generation at IKI-C. Proportion of Kan^S clones obtained in each independent lineage (symbolized by a flake) of WT, ∆kuA and ∆ligCD contexts. For each of the 50 lineages, an average of 230 clones was analyzed by replica-plating with and without kanamycin.