Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 27;8:5272. doi: 10.1038/s41598-018-23622-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fates of the chromosome structure following DSB and arm replacement. Red and blue boxes represent left and right replichores of the chromosome respectively. Grey arrows and the grey oval represent the terminal inverted repeats and the terminal proteins attached to them. HR2-L and HR2-R are symbolized as salmon pink boxes. The hybrid HR1 and HR2 sequences are labelled HR1-L/R and HR2-L/R respectively. HR3 and HR4 are symbolized as purple boxes. The AUD90 locus, susceptible to DNA amplification is marked out by light blue boxes between brackets. The tap-tpg locus encoding the terminal associated protein and the terminal protein respectively is represented by a black star. The yellow PacMan symbolizes the accessibility of the chromosome end to nuclease activities. After DSB induction at IKI-L, DNA resection progresses towards the inner parts of the chromosome concomitantly with the loss of the distal part of the arm. In case (1), DNA resection does not reach the tap-tpg locus, and arm replacement occurs by HR between HR1-R and HR1-L. Since tap-tpg locus is preserved, the linear configuration of the rearranged chromosome is stable. In case (2), DNA resection gets past tap-tpg locus and arm replacement is initiated by HR between HR2-R and HR2-L. The tap-tpg being deleted, this chromosome is unstable and irremediably leads to stabilization through circularization by different ways involving either HR3, or HR4 or an amplified sequence as previously shown.