Fig. 2. Simultaneous detection of multiple DNA glycosylases by TIRF-based single-molecule imaging in the absence (A and E) and presence of hOGG1 (B and F), hAAG (C and G) and both hOGG1 and hAAG (D and H). The Cy3 fluorescence signals are shown in green, and the Cy5 fluorescence signals are shown in red. The Cy3-labeled molecular beacon (0.3 μM), the Cy5-labeled molecular beacon (0.3 μM), hOGG1 (0.1 U μL^–1), hAAG (0.1 U μL^–1) and APE1 (0.1 U μL^–1) were used in this research. The scale bar is 5 μm.