FIG 1.

Flowchart of sample disposition after reevaluation of data with the 2017 software update. Footnotes a to g in the flowchart include additional information about the categories. For footnote a, the 560 excluded samples include the following reasons for exclusion: deviation from the protocol (n = 216), experiments halted (n = 26), experiments never run (n = 15), bottle received >8 h after positive result (n = 31), Gram staining shows no organism (n = 24), isolate not received at the reference laboratory (n = 18), isolate received more than 4 days after medium preparation (n = 3), ID reference growth failure (n = 29), nonpure isolate (n = 169), ID reference purity plate failure (n = 6), invalid ID reference result (n = 1), and Accelerate Pheno system run state not “complete” (n = 22). For footnote b with monomicrobic, a single on-panel organism was reported. For footnote c with polymicrobic, this category includes polymicrobial samples where the Accelerate PhenoTest BC kit ID results exactly match the reference results. For footnote d with false-positive, the false-positive category includes monomicrobial or polymicrobial runs containing any false-positive result(s). For footnote e with indeterminate, all indeterminate samples had only indeterminate/negative results. For footnote f with unresolved for fresh samples, of the 48 fresh unresolved false-positive results, 43 showed genus-level agreement, while the remaining five were one S. aureus called CoNS, one S. aureus plus Pantoea species mix called Klebsiella spp., one Pseudomonas putida called Citrobacter spp., one Lactococcus raffinolactis called Streptococcus spp., and one Streptococcus dysgalactiae subsp. equisimilis called E. faecium by the Accelerate Pheno system. For footnote g with unresolved for seeded samples, of the 24 seeded samples containing unresolved false-positive results, 15 showed genus-level agreement, one sample gave two false-positive results, while the remaining nine were one Pantoea sample called Enterobacter spp., one C. koseri sample called Citrobacter spp. plus Proteus spp., two C. koseri samples called Citrobacter spp. plus Klebsiella spp., one C. freundii sample called Citrobacter spp. plus Enterobacter spp., one C. koseri sample called Citrobacter spp. plus E. faecium plus Klebsiella spp., one E. cloacae complex sample called Enterobacter spp. plus P. aeruginosa, one E. faecalis sample called E. faecalis plus E. faecium, and one Streptococcus pyogenes sample called Streptococcus spp. plus E. faecium by the Accelerate Pheno system.