TABLE 1.

Four different methods of serogrouping D. nodosus compared at the flock level^a

Flock	No. of sheep examined	No. of sheep sampled	No. of feet sampled	Culture based serogrouping (pure cultures)				Culture independent serogrouping (lesion swabs)
				Slide agglutination		fimA PCR		fimA PCR on mSTM swabs		fimA PCR on LB swabs
				No. of isolates tested	Serogroup(s) detected	No. of isolates tested	Serogroup(s) detected	No. of swabs tested	Serogroup(s) detected	No. of swabs tested	Serogroup(s) detected
1	51	24	24	25	A, (D), (G)	25	A	24	A	24	A
2	52	42	50	46	A, B, H, I, (D), (F), (G)	36	A, B, H, I	50	A, B, G, H, I	50	A, B, G, H, I
3	42	20	20	36	C, D, E, G, I, (H), (M)	34	C, D, E, G, H, I	20	D, G, H	20	D, E, G, H
4	33	20	20	36	A, B, E, H, I, (C), (D)	36	A, B, E, H, I	20	A, B, E, G, H	20	A, B, E, G, H, I
5	34	20	20	33	A, B, D, G, H, (C), (M)	33	A, E, D, G, H, I	20	A, B, E, G, H	20	A, B, D, E, G, H
6	28	20	20	19	A, B, (D), (E), (H), (I)	19	B	20	A, B	20	A, B
7	13	13	13	13	A, B	13	B	13	A, B	13	A, B
8	27	20	20	20	E, G, H, (B)	20	E, G, H	20	G, H	20	E, G, H
9	20	20	20	14	A, B, E, G, H	14	A, B, E, H	20	A, B, E	20	A, B, E, G
10	12	12	12	11	G	10	G	0	Not tested	12	E, F, G, H
11	26	25	25	22	C, H, (B), (D), (E), (F)	21	H	0	Not tested	25	A, C, D, E, G, H
12	26	25	25	15	E, (C), (G)	14	E	0	Not tested	25	E
Total	364	261	269	290		275		207		269

^aAmbiguous slide agglutination test results are shown in parentheses. mSTM, modified Stuart's transport medium; LB, lysis buffer.