Figure 3.

Chromatin association of P. abyssi Cdc6 (PAB2265) and MCM (PAB2373). (A) Expression patterns of P. abyssi Cdc6 and MCM were investigated by immunoblotting of 10% or 8%, respectively, SDS/PAGE gels after various times after inoculation. Puromycin was added after 4 h of growth. The cell densities of samples (10⁷ cells per milliliter) are indicated below each lane. As whole-cell lysates were directly analyzed, the results should be considered semiquantitative. (B) Chromatin binding of P. abyssi Cdc6 and MCM was investigated by separating cell lysates into soluble (S) and chromatin-enriched (P) fractions. Where indicated, puromycin (200 μg/ml)-treated or stationary phase cells were used for assay. All samples were extracted from a comparable amount of cells. Protein amounts analyzed per lane are indicated below each lane in micrograms.