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A schematic model of archaeal initiation complexes at the replication origin in replicating (exponential-phase culture) and nonreplicating (puromycin-treated samples) P. abyssi cells. The shaded box and arrow indicate the P. abyssi cdc6 gene and its transcriptional start in the vicinity of the mapped oriC. Cdc45 and Cdt1 missing in Archaea are essential for replication initiation in Eukarya. RC, replication complex.
