Fig. 5.

Subcellular localization for GIRK channel subunits in PCs. Distribution of immunoparticles for the GIRK3 and GIRK2 subunits using the SDS-FRL technique in wild-type (WT) and GIRK knockout (KO) mice. a, b Immunoparticles for the GIRK3 subunit are detected in dendritic spines (s) and dendritic shafts of oblique dendrites (oDen) of PCs. c The antibody specificity was controlled and confirmed in replicas of GIRK3 KO mice that were free of any immunolabelling. d, e Immunoparticles for the GIRK2 subunit are also detected in dendritic spines (s) and dendritic shafts (Den) of PCs, but at lower frequency than GIRK3. f The immunogold labelling for GIRK2 was abolished in the GIRK2 KO mice. Scale bars: a–f 0.2 μm