Figure 2.

Pharmacological examination of purinergic receptors expressed in TRPM5-MCs. Plot of percentage of cells responding to each stimulus: 100 μM ATP, 100 μM ATP in the presence of 5 μM pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid (PPADS), 100 μM ATP in Ca²⁺-free saline, 100 μM UTP, 100 μM ADP, 100 μM AMP and 100 μM adenosine. Numbers in the bars are number of responded cells/number of cells tested. *p < 0.05, **p < 0.01, ***p < 0.001, Fisher’s exact test. Inset: representative responses to each stimulus shown as changes in Ca²⁺ levels. Note: although fewer cells were responsive to Ca²⁺-free ATP and adenosine than to ATP, their response amplitudes are close to the response to ATP.