Table 2.

Binding properties of T1-11 toward A_2AR in the absence or presence of D₂R.

cDNA	Ki values (μM)
	CON	QP
A2AR	4.0 ± 1.6	3.8 ± 1.2
A2AR + D2R	2.7 ± 0.5	4.4 ± 0.9

NSC34 cells were transfected with A_2AR in the presence or absence of D₂R for 44 h, followed by a 4-h incubation with ADA (1 U/ml) at 37°C to remove endogenous adenosine. The membrane lysates were collected and subjected to the receptor binding assay to compete ³H-CGS21680 in the absence or presence of QP (1 μM), as described in the Supplemental Method. The data is presented the mean ± SEM of three independent experiments.