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. 2018 Mar 20;9:93. doi: 10.3389/fgene.2018.00093

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Copyright © 2018 Riad, Elhelw, Shawer, El-Ekiaby, Salah, Zekri, Esmat, Amleh and Abdelaziz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Predicted alignment and luciferase reporter assay. (A) miR-182 targets CLDN1 3′UTR at position 1120 with a mirSVR score of −1.918 as predicted by microRNA.org. (B) A decline in luciferase activity is evident in Huh7 cells co-transfected with miR-182 mimic and pmirGLO vector harboring the wild type (WT) insert (N = 6) (p = 0.0035^**), compared to WT untransfected cells (N = 6). However, miR-182 mimic does not alter luciferase activity in either empty pmirGLO (N = 9) or pmirGLO vector harboring the mutant type (MT) insert (N = 9), each compared to un-mimicked cells (N = 9). Comparing firefly luciferase activity in miR-182 transfected cells containing wild-type to those containing mutant type 3′UTR, a moderate but significant reduction is also evident (p-value of 0.0442^*). ^**p < 0.01, ^*p < 0.05 and ns, statistically not significant.