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. 2018 Mar 20;9:93. doi: 10.3389/fgene.2018.00093

Figure 4.

Figure 4

Impact of miR-155 and miR-182 on CLDN1 expression in naïve Huh7 cells. (A) Both miR-155 (p = 0.0173*) and miR-182 mimics (p = 0.0071**) significantly reduce CLDN1 mRNA expression compared to untransfected cells). Antagomirs against miR-155 and miR-182 restore CLDN1 mRNA expression to a level not significantly different than the untransfected cells. Knocking down CLDN1 using siRNAs suppresses CLDN1 mRNA levels in transfected Huh7 cells (p = 0.0007***) compared to untransfected cells. Relative Quantitation (RQ) is used for expression of the results. (B) Western blot analysis shows that cells transfected with miR-155 (p = 0.0014**) and miR-182 mimics (p = 0.0308*) show significantly lower levels of CLDN1 protein as compared to untransfected cells. CLDN1 siRNA (p = 0.0195*) also reduce CLDN1 protein levels compared to untransfected cells. ***p < 0.001, **p < 0.01, *p < 0.05 and ns, statistically not significant.