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[35S]CaM binding to wt-RyR and C3635A RyR. Membrane fractions prepared from HEK293 cells and expressing wt-RyR1 (white bars) or C3635A mutant RyR1 (black bars) were incubated with 5 or 15 nM [35S]CaM and either 5 mM EGTA or 100 μM Ca2+. The ratios of [35S]CaM-binding values to maximal binding values of [3H]ryanodine are shown. Maximal values of [3H]ryanodine binding were determined as described under Experimental Procedures. Data are the means ± SD of three experiments.
