FIGURE 8.

Roles of Aacsn5 in nutrient utilization and toxin production during development. (A) Growth of the wildtype and ΔAacsn5 on different carbon or nitrogen sources. Mycelial plugs were taken from the wildtype and ΔAacsn5 and grown individually on Czapek’s medium with the glucose, sucrose, cellulose, hemicellulose, pectin, or citrus leaf as the sole carbon source or, with NaNO₃, yeast extract, peptone, urea, acetamide, or phenylalanine as the sole nitrogen source at 25°C for 6 days. (B) Necrotic lesions appearing on detached citrus leaves inoculated by spraying with the crude ACT toxin extracts from both the wildtype and ΔAacsn5 in the axenic culture.