Figure 6.

Cardiac hypertrophy analysis. (A) Hearts were removed from male mice (6 month-old, n = 5/genotype), and their weight was normalized to tibial length as a measure of cardiac hypertrophy. (B) qRT-PCR analysis of mRNA of markers of hypertrophy in heart homogenates. These include β-Mhc (i), Anp (ii) and Bnp (iii) (n = 5/genotype in triplicate). (C) H&E stain of heart sections from mice (3 sections/mouse). In the accompanying graph, the average myocyte area was quantitated under the microscope at 20× magnification. (D) mRNA analysis of markers of cell proliferation in heart homogenates. These include Pcna (i), c-Jun (ii), and c-Fos (iii). Analysis was performed relative to Gapdh and expressed as mean ± SEM (n = 5/genotype in triplicate). In (A, B and D), values are expressed as mean ± SEM. *P ≤ 0.05 versus Cc1^+/+ and †P ≤ 0.05 Cc1^{−/−xliver+} versus Cc1^−/− mice.