Figure 1.

Changes in plasma ghrelin with an acute single bout of exercise. A. Schematic showing the timeline of the experiment. Food was removed from the mice for a total duration of 6 h and the mice were either sedentary (Sed), exercised in the 6^th h of food restriction (“6^thh EX”) or exercised in the 2^ndh of food restriction (“2^ndh EX”). The single bout of exercise lasted for 1 h, with the speed and duration of the treadmill run as indicated in the inset and described in Materials and Methods. (B) Plasma acyl-ghrelin, (C) total ghrelin, and corresponding (D) blood glucose in wild-type (Wt) and GHSR-null littermates subjected to the 3 test conditions at the end of the 6 h experimental period. Data was analyzed by two-way ANOVA followed by Sidak post hoc multiple comparison test. n.s. – no significant difference, ****p < 0.001, significant difference. n = 12 per group. Data represented as mean ± S.E.M.