Figure 2.

Feeding response post-exercise in wild-type and GHSR-null mice. A. Body weight of Wt and GHSR-null mice submitted to the HIIE protocol for studying the changes in flood intake due to lack of GHSRs. Food intake following re-introduction of standard chow to Wt or GHSR-null mice following a 6 h period of food withdrawal during which mice were either sedentary (Sed; B), exercised in the 6th h of food restriction (6^thh EX; C), or exercised in the 2nd h of food restriction (2^ndh EX; D). Data in B-D were analyzed by repeated measures two-way ANOVA followed by Sidak post hoc multiple comparison test to compare differences in food intake between genotypes at each time point. The food intake data for Wt mice represented in panels B, C, and D are combined and re-analyzed in panel E. The food intake data for GHSR-null mice represented in panels B, C, and D are combined and re-analyzed in panel F. Data in E and F were analyzed by repeated measures two-way ANOVA followed by Sidak post hoc multiple comparison test to compare the overall differences in food intake due to exercise. One mouse in the GHSR-null group was excluded as an outlier based on Grubb's test. n.s. – no significant difference, *p < 0.05, **p < 0.01, ****p < 0.001, significant difference. n = 5–6 per group. Data represented as mean ± S.E.M.