Figure 7.

Effect of exercise on gastrocnemius glycogen content and muscle activity markers in wild-type and GHSR-nulls. Changes in gastrocnemius (A) glycogen content, (B) AMPK activity as measured by Thr172 phosphorylation, (C) ACC activity as measured by Ser79 phosphorylation, and (D) mitochondrial COX IV protein expression, in exercised mice. Bar graphs in B, C and D illustrate the western blot densities normalized to the average values for sedentary Wt mice. Representative western blots are shown for bar graphs B, C and D. Data were analyzed by two-way ANOVA followed by Sidak post hoc multiple comparison test. n.s. – no significant difference, ***p < 0.005, significant difference. n = 9–12 per group. Data represented as mean ± S.E.M.