Figure 5.

IGFR knockout in astrocytes increases maximal respiration by Seahorse. (A) Knockout of IGFR (CRE) in astrocytes significantly decreased oxygen consumption. (B) OCR was reduced significantly in several stages of respiration in IGFR knockout (CRE) astrocytes including, basal, ATP-linked, proton-leak and non-mitochondrial respiration. Maximal respiration was reduced but not significantly. (C) Oligomycin-induced extracellular acidification (ECAR) was significantly decreased in IGFR-KO, while basal ECAR was not different between groups. (D) Basal and maximal OCR to ECAR ratio depicts lower induction of response with FCCP in CRE astrocytes compared to control. Data are represented as the Mean ± SEM (n = 7/group), *P < 0.05.