Skip to main content
. 2018 Feb 3;9:28–42. doi: 10.1016/j.molmet.2018.01.017

Figure 3.

Figure 3

Hit validation in brown adipocytes identified five compounds that stabilize PGC-1α1 protein and activate Ucp1 transcription. Fully differentiated brown adipocytes were treated for 8 h with a compound (10 μM), a positive control, isoproterenol (10 μM) 4 h or 8 h, MG132 8 h (10 μM), or with a negative control D = DMSO and harvested for RNA and protein. ∗indicates that in that lane a protein molecular size marker was loaded together with the DMSO-treated negative control extract. (A) Ucp1 expression in brown adipocytes for 79 validated compounds. (B) Gene expression panels for five select compounds and for controls, isoproterenol and MG132. (C) PGC-1α1 protein stabilization by western blot for a selection of validated compounds. Compounds that were chosen for further analysis are shown in bold. Isoproterenol 4 h (i4) or 8 h (i8). m, protein molecular size marker. Lower panel of (C) shows Ponceau S stained PVDF membranes. Results in A and B are mean ± SEM of a single experiment.