Figure 5.

Compound treatment increased basal mitochondrial respiration trough increased proton leak in brown adipocytes. Brown adipocytes were treated for 8 h with 10 μM of AM31 (A), AM73 (B), AM79 (C), AM80 (D), AM89 (E) or 10 μM of isoproterenol (F) after which oxygen consumption rates (OCRs) were measured before and after addition of oligomycin (blocks ATP synthesis), FCCP (=uncouples respiration, leads to maximal respiration) and antimycin (=inhibitor of complex III and of mitochondrial respiration). Data shown is the average ± SEM of four independent experiments. For statistical analysis, each condition (basal/oligomycin/FCCP/antimycin) was analyzed individually. Different groups were analyzed by one-way ANOVA. ^∗ indicates a p-value < 0.5.