Figure 3.

Co-staining of TUNEL (green) and DAPI (blue) in the perihematomal cerebral cortex at 24 h after ICH. TUNEL-positive cells were barely detected in the sham group but were widely distributed in the perihematomal area after ICH. r-OPN decreased the amounts of TUNEL-positive cells, which was blocked by wortmannin. Scale bar 50 μm (A). ICH increased the number of TUNEL-positive cells compared with sham group. r-OPN remarkably attenuated the number of TUNEL-positive cells, whereas wortmannin combined with r-OPN reversed the decreasing trend compared with only r-OPN treatment (B). Data are presented as the mean ±SEM (* P<0.05 compared with sham, ^# P<0.05 compared with vehicle, ^& P<0.05 compared with r-OPN, n=4–5/group).