Figure 7.

TALK-1 KO α-cells exhibit altered Ca²⁺dynamics only in intact islets. (A) Recordings of intracellular Ca²⁺ responses in WT α-cells, in islets expressing the genetically encoded Ca²⁺ indicator GCaMP3 specifically in α-cells (representative of islet α-cells from N = 3 mice). (B) Recordings of intracellular Ca²⁺ responses in TALK-1 KO α-cells, in islets expressing the genetically encoded Ca²⁺ indicator GCaMP3 specifically in α-cells (representative of islet α-cells from N = 3 mice). (C) Recordings of intracellular Ca²⁺ responses in single WT α-cells expressing the genetically encoded Ca²⁺ indicator GCaMP3 (representative of α-cells from N = 3 mice). (D) Recordings of intracellular Ca²⁺ responses in single TALK-1 KO α-cells expressing the genetically encoded Ca²⁺ indicator GCaMP3 (representative of α-cells from N = 3 mice). (E and F) Comparison of percent oscillating α-cells and Ca²⁺ AUC determined from WT and TALK-1 KO α-cells under the indicated conditions (N = 3 mice per genotype). (G) Islets were incubated for 1 h with the indicated treatments (SSTR2 antagonist: 500 nM CYN154806); N = 10 mice per genotype (glucose only); 3 mice per genotype (+CYN154806); *P < 0.05.