Figure 7.

Steps and equilibria considered in our quantitative model of chemical primer extension [34]. The model considers the binding of the activated monomer with its leaving group (LG) to the primer–template complex in the form of the dissociation constant (K_d). It takes into account the rate of hydrolysis with the corresponding rate constant (k_h), the binding equilibrium for the hydrolyzed monomer that acts as inhibitor (K_dh), and it assumes a single rate-limiting chemical step (k_cov); B, B' = nucleobase = OH for RNA.