Fig. 2.

Intracellular localization of NS1 during WT and mutant infection in MDCK cells. MDCK cells were infected with the WT and mutated virus (S42P, D92E, S42P/D92E) at an MOI of 0.001. At 6(a), 12(b), and 24(c) hpi, the cells were fixed and stained with anti-NS1 antibody and DAPI. The localizations of NS1 proteins were visualized using a Nikon Eclipse 80i fluorescence microscope. NS1: stained with FITC-labelled goat Anti-Mouse IgG (green); Nuclei: stained with DAPI (blue); Merge: merged image of double immunofluorescent staining of the same field