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. 2018 Mar 27;19:105. doi: 10.1186/s12859-018-2115-4

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Workflow. Steps: 1. CpGo/e ratios are calculated for the sequences to be analyzed (in our case dbEST) using CpGoe.pl. 2. Removal of outliers (first step of KDEanalysis.r). 3. Mode detection (second step of KDEanalysis.r)