Fig. 5.

NOL4 and TMEM97 are miR-152-3p targets a Schematic representation of the miR-152-3p’s MRE in NOL4 and TMEM97. Luciferase activity in HEK293Ta cells co-transfected with reporter constructs containing NOL4 MRE or TMEM97 MRE and either pre-miR-152-3p or pre-miR-NC. b Validation of candidate sgRNAs for TMEM97 by a competitive proliferation assay in LNCaP cells. c SgRNA NT indicates a nontargeting. GFP levels were measured by flow cytometry. Values are normalized to day 0 (T = 0). 3′UTR: 3′ Untranslated Region; MRE: miRNA response element Mann-Whitney U test: *p < 0.05, **p < 0.01, ***p < 0.001