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. 2017 Apr 7;73(4):350–360. doi: 10.1136/thoraxjnl-2016-208977

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This is an Open Access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 4.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. See: http://creativecommons.org/licenses/by/4.0/

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Activation of retained lung monocytes and neutrophils during ischaemia-reperfusion (I/R). Monocyte and neutrophil activation during I/R was indicated by changes in expression of their respective surface activation markers relative to 2 hours of perfusion only, with values for those from untreated (non-surgical) control mice indicated by a dotted line. Ly6C^High monocyte activation was indicated by L-selectin shedding and CD86 upregulation (A); Ly6C^Low monocytes and interstitial macrophages by increased CD86 expression (B and C); and neutrophils by L-selectin shedding and increased surface CD11b expression (D). Data are displayed as mean±SD and analysed by t tests. n=4–8, *p<0.05, **p<0.01, ***p<0.001.