Figure 5.

Adoptive transfer of monocytes restores ischaemia-reperfusion (I/R)-induced lung injury in monocyte-depleted lungs. Isolated lungs from monocyte-depleted mice were infused slowly (1 min) with normal blood-derived monocytes and recirculated for 10 min prior to initiation of the ischaemic period. At the end of I/R, Ly6C^High monocyte numbers were found to be elevated in infused lungs (A). Lung wet:dry weight ratios (B) and bronchoalveolar lavage (BAL) protein levels (C) were increased in monocyte-infused lungs, indicating monocyte-dependent IR injury. Post-I/R treatment values obtained in previous experiments in normal (non-monocyte-depleted) I/R mice are indicated by a dotted line. Data are analysed by t tests (A and B; mean±SD) or by Mann–Whitney U tests (C; median±IQR). n=4, *p<0.05. clod-lipo, clodronate-liposome.