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. 2017 Apr 7;73(4):350–360. doi: 10.1136/thoraxjnl-2016-208977

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This is an Open Access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 4.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. See: http://creativecommons.org/licenses/by/4.0/

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Identification of monocyte subsets in human donor lung grafts. Single cell suspensions prepared from donor lung tissue were antibody stained for surface markers, and then fixed and permeabilised for intracellular detection of the monocyte and neutrophil S100A8/A9 antigen using the 27E10 monoclonal antibody (A). Classical subset monocytes were identified in lungs as 27E10^High, CD66b– (R1) and CD14+, CD16– (R3) events. Granulocytes were identified as 27E10^High, CD66b+ events (R2). Using the same staining protocol, comparable staining characteristics were observed for monocytes in healthy volunteer whole blood (B), including similar expression levels of HLA-DR and CCR2.