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. 2018 Mar 27;18:330. doi: 10.1186/s12885-018-4250-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — D5D-KD and DGLA supplementation enhance the efficacy of 5-FU on growth of 4 T1 cells. a Colony formation assay of D5D-KD 4 T1 cells or control siRNA transfected cells at 10 days with treatment of 5-FU (20 μM) or 5-FU + DGLA (100 μM) for 48 h. Note, D5D-KD and NC-si control cells without DGLA and 5-FU treatment (shown in Fig. 3) were used to calculate survival fractions; and b Cell apoptosis was examined via flow cytometry after D5D-KD 4 T1 cells were treated with DGLA (100 μM), 5-FU (20 μM), or 5-FU + DGLA for 48 h, followed by Annexin V-FITC/PI double staining Data represent as mean ± standard deviation (*: significant difference vs. control with p < 0.05; and #: significant difference vs. 5-FU group with p < 0.05 from n ≥ 3)