Table 3.
Serologic analyses of sera obtained 3 days before (Pre) and 14 days post-infection (dpi)
| Virus | Pig ID | HAI titera | S:N of ELISAb | ||||
|---|---|---|---|---|---|---|---|
| Pre | 14 dpi | Pre | 14 dpi | ||||
| Yam10(H1N1) | 1 | < 10 | 80 | 0.95 | – | 0.72 | – |
| 2 | < 10 | 80 | 0.86 | – | 0.88 | – | |
| 3 | < 10 | 80 | 0.97 | – | 0.68 | – | |
| 4 | < 10 | 80 | 0.88 | – | 0.85 | – | |
| Kag15(H1N1) | 1 | < 10 | 20 | 0.96 | – | 0.73 | – |
| 2 | < 10 | 160 | 0.89 | – | 0.31 | + | |
| 3 | < 10 | 160 | 0.91 | – | 0.61 | – | |
| 4 | < 10 | 80 | 0.93 | – | 0.53 | + | |
| Toc08(H1N2) | 1 | < 10 | 20 | 1.04 | – | 0.76 | – |
| 2 | < 10 | 20 | 1.02 | – | 0.62 | – | |
| 3 | < 10 | 10 | 0.91 | – | 0.66 | – | |
| 4 | < 10 | 10 | 1.06 | – | 0.66 | – | |
| Toc12(H1N2) | 1 | < 10 | 80 | 0.85 | – | 0.71 | – |
| 2 | < 10 | 80 | 0.78 | – | 0.56 | + | |
| 3 | < 10 | 160 | 0.96 | – | 0.39 | + | |
| 4 | < 10 | 80 | 0.90 | – | 0.52 | + | |
| Chi15(H1N1) | 1 | < 10 | 160 | 1.08 | – | 0.59 | + |
| 2 | < 10 | 80 | 0.91 | – | 0.56 | + | |
| 3 | < 10 | 160 | 0.87 | – | 0.55 | + | |
| 4 | < 10 | 80 | 0.86 | – | 0.56 | + | |
| Nag00(H3N2) | 1 | < 10 | 160 | 0.94 | – | 0.79 | – |
| 2 | < 10 | 80 | 0.71 | – | 0.74 | – | |
| 3 | < 10 | 80 | 0.70 | – | 0.72 | – | |
| 4 | < 10 | 160 | 0.84 | – | 0.44 | + | |
| Miy13(H3N2) | 1 | < 10 | 320 | 0.72 | – | 0.78 | – |
| 2 | < 10 | 320 | 1.02 | – | 0.78 | – | |
| 3 | < 10 | 160 | 0.70 | – | 0.59 | + | |
| 4 | < 10 | 160 | 0.73 | – | 0.72 | – | |
| Yok11(H3N2) | 1 | < 10 | 320 | 0.93 | – | 0.74 | – |
| 2 | < 10 | 80 | 0.93 | – | 0.70 | – | |
| 3 | < 10 | 160 | 0.95 | – | 0.62 | – | |
| 4 | < 10 | 160 | 0.82 | – | 0.47 | + | |
| Min06(H3N2) | 1 | < 10 | 320 | 1.00 | – | 0.72 | – |
| 2 | < 10 | 320 | 0.68 | – | 0.96 | – | |
| 3 | < 10 | 160 | 0.93 | – | 0.96 | – | |
| 4 | < 10 | 80 | 0.75 | – | 0.49 | + | |
| Control | 1 | < 10 | < 10 | 0.84 | – | 0.95 | – |
| 2 | < 10 | < 10 | 0.82 | – | 0.93 | – | |
| 3 | < 10 | < 10 | 0.82 | – | 0.95 | – | |
| 4 | < 10 | < 10 | 0.86 | – | 0.95 | – | |
aHomologous viruses were used as antigens in each group. Sera from control pigs were tested against classical H1 (Chi15), A(H1N1)pdm09v (Kag15), and triple-reassortant (Yok11) IAVs-S as antigens
bFor swine sera, a sample:negative (S:N) ratio < 0.6 was considered positive (+; −, negative), according to the manufacturer’s instructions