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. 2018 Mar 27;37:67. doi: 10.1186/s13046-018-0721-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Galectin-1-mediated adhesion to fibronectin is inhibited by PTX008. a-c Representative analysis of (a) US7, (b) LAX56 or (c) LAX57 cells adhering to fibronectin (left panels) or poly-L-lysine coated plates (middle panels) in the presence of increasing concentrations of PTX008 or 5 and 50 mM lactose. Error bars: standard deviation. Cell surface staining using FACS (Genetex antibodies) for Galectin-1 on these samples (right panels). Numbers, percentage of cells in the upper left quadrants. *p≤0.05 (95% CI, 1-way ANOVA). d Summary fold difference in adhesion of LAX56 and LAX57 to fibronectin and poly-L-lysine coated plates. Percent adhesion was normalized to DMSO control for 3 replicate experiments with 3 or more replicate wells. Error bars, standard error of mean (**p<0.01, 1-way ANOVA)