Skip to main content
. 2018 Mar 20;9:364. doi: 10.3389/fpls.2018.00364

FIGURE 4.

FIGURE 4

Map-based cloning of RFS and phylogenetic analysis of the RFS proteins in other plants. (A) Genetic mapping of the RFS gene using SSR and STS markers. (B) Physical mapping of the RFS gene. The genomic region contains five expressed genes (gray and black boxes). The genomic structure of RFS, comprising 11 exons and 10 introns, is indicated. A deletion mutation (SFQ 811–813) was found in exon 6 of RFS in the rfs-1 mutant. (C) Nucleotide mutations result in deletion of the amino acids SFQ (asterisks) in the SNF2 family N-terminal domain (gray line). (D) The phylogenetic tree was constructed by the neighbor-joining method with MEGA (ver 5.1) program. Branch numbers represent percentage of bootstrap values in 1000 sampling replicates and the scale indicates branch length.