Fig. 3. IL-6 and NOTCH1 are mediators of CK5-related differentiation in DAC-treated cells.

a Bladder tumor cells were treated with DAC every 48 h for 5 days. CK5 protein expression is downregulated in DAC-treated cells on day 5. GAPDH was used as loading control. The western blot is a representative of triplicate experiments. b Western blots from triplicate experiments were quantitated by ImageJ. CK5 values were normalized to Tubulin from each condition and plotted on the graph. c CK5 protein expression is downregulated in ICN1 overexpressing cells. GAPDH was used as loading control. The western blot is a representative of triplicate experiments. d Western blots from triplicate experiments were quantitated by ImageJ. CK5 values were normalized to Tubulin from control and ICN1 overexpressing cells and plotted on the graph. e The addition of 50ng/ml IL-6 reduced CK5 expression in HT and B02 cells. The addition of a monoclonal antibody against IL-6 rescued CK5 expression only in DAC-treated B02 cells. GAPDH was used as loading control. The western blot is a representative of triplicate experiments. f Western blots from triplicate experiments were quantitated by ImageJ. CK5 values were normalized to GAPDH from each condition and plotted on the graph.