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. 2017 Dec 13;8(12):3215. doi: 10.1038/s41419-017-0053-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a H1299 cells were transfected with pXJ40-FLAG, pXJ40-FLAG-Bcl2, or pXJ40-FLAG-Bcl2(S70A) before being infected with IBV at MOI~2 or being mock infected. The cells were harvested at indicated time points and were subjected to Western blot analysis using the indicated antibodies. Beta-tubulin was included as loading control. Sizes of protein ladders in kDa were indicated on the left. PARP cleavage was determined as in Fig. 3b. The experiment was repeated three times with similar results, and result of one representative experiment is shown. b Huh-7 cells were transfected and infected as in a. Western blot analysis and quantification of PARP cleavage was determined as in a. The experiment was repeated three times with similar results, and the result of one representative experiment is shown.