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. 2017 Dec 13;8(12):3210. doi: 10.1038/s41419-017-0051-2

Fig. 4. Control of spherogenicity by TGF-β or c-MET pathway.

Fig. 4

ZH-161 or ZH-305 cells were plated at 300 cells/well in NB medium in the absence or presence of SD-208 (1 µM) or TGF-β2 (10 ng/ml) or SD-208 combined with TGF-β2 in 96-well plates. c-MET shRNA expressing or EMD1214063 (200 nM)-treated ZH-161 or ZH-305. Sphere formation was assessed at day 21 in triplicates (*p < 0.05, effects of TGF-β2 compared to control, # p < 0.05, effect of TGF-β2 and SD-208 co-treatment compared to TGF-β2 alone; ω p < 0.05, effect of EMD1214063 compared to control, θ p < 0.05, effect of c-MET shRNA compared to control). Spherogenicity was calculated based on the number of spheres and average size of the spheres as indicated (upper panels). Representative photomicrographs showing sphere formation in each condition for ZH-161 (middle panels) and ZH-305 (lower panels). The photomicrographs were taken using a 5X objective