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. 2017 Dec 11;8(12):3200. doi: 10.1038/s41419-017-0041-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Morphology of hiPSCs before MSC induction was examined by immunofluorescence staining. b Flow cytometric analysis of surface markers of hiPSC-MSCs. c Left: TEM images of EVs derived from hiPSC-MSCs (scale bar, 100 nm); middle: the sizes of hiPSC-MSCs-derived EVs (mean: 135 nm); right: western blot for Alix, CD80, and CD63 in hiPSC-MSCs-derived EVs. Medium alone is the control; d a standard curve indicated the relation between the protein content and the EVs number