Fig. 2. Inhibition of caspase activity prevents ROS-induced decrease in NHLRC2 protein levels in HCT116 cells.

a Immunoblotting analysis of NHLRC2 and caspases in HCT116 cells treated with tBHP and z-VAD-fmk. b Caspase-8 activity in HCT116 cells treated with tBHP. The results are shown as the values relative to 0 µM tBHP cells. c, d Percentages of cells that underwent apoptosis for HCT116 cells treated with tBHP and z-VAD-fmk. c Numbers adjacent to the outline indicate the percentage of cells in each area. d The sum of annexin V⁺PI⁻ and annexin V⁺PI⁺ populations in c is represented as the percentage of annexin V⁺ cells. Data represent the mean ± SD based on three independent experiments. *P < 0.05. a–d Data are representative of three independent experiments