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. 2017 Dec 14;8(12):3218. doi: 10.1038/s41419-017-0006-7

Fig. 7. shRNA-mediated knockdown of NHLRC2 increases the susceptibility of HCT116 cells to ROS-induced apoptosis.

Fig. 7

a Immunoblotting analysis of NHLRC2 in HCT116 cells transduced with retrovirus for shRNAs for control (sh-Control) or NHLRC2 (sh-NHLRC2-3 and -5). b–d Analyses of cell proliferation b and apoptosis c, d in HCT116 cells transduced with retrovirus for sh-Control, sh-NHLRC2-3 or -5. b *P < 0.05. c Numbers adjacent to the outline indicate the percentage of cells in each area. d The sum of annexin V+PI− and annexin V+PI+ populations in c is represented as the percentage of annexin V+ cells. Data represent the mean ± SD based on three independent experiments. *P < 0.05. a–d Data are representative of three independent experiments