Fig. 7.

eIF2α overexpression suppressed the HRD1 protection of HKC-8 cells against apoptosis. a Treatment with palmitic acid (PA) alone and forced expression of eIF2α markedly increased BAX expression, but decreased Bcl-2 expression. Ad-HRD1 infection significantly suppressed BAX and restored Bcl-2 expression. However, the HRD1 protection induced by Ad-HRD1 infection in PA-treated HKC-8 cells was blunted by transfection with Myc-eIF2α. b Quantitation of western blot data for protein expressions as in (a), normalized to actin expression. Bars are means ± S.E. from three independent experiments. *P  < 0.05. c Schematic diagram showing the promotion of ubiquitylation and degradation of eIF2α by HRD1 benefit renal apoptosis