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. 2017 Dec 13;8(12):3209. doi: 10.1038/s41419-017-0034-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Sensory neurons derived from rat DRG (5 × 10⁴ cells/cm²) and rat bone marrow MSCs (10⁴ cells/cm²) were cocultured in microfluidic devices for 7 days. DRG neurons were maintained in DMEM supplemented with 2% (v/v) B-27 and 1 μM AraC; MSCs were incubated in OIM composed of DMEM-low glucose with 10% (v/v) FBS, 1 × 10⁻⁹ M dexamethasone, 10 mM β-glycerophosphate, and 50 μg/mL ascorbic acid. a The DNA concentration of MSCs was determined at 4 and 7 days of coculture by CyQUANT™ Cell Proliferation Assay. b The relative metabolic activity of MSCs was measured at 4 and 7 days of coculture by resazurin-based assay and normalized to the monoculture levels on day 4. Data expressed as mean ± SD. (n) indicates the total number of samples for each group. **p < 0.01 statistically different from monoculture. The results represent three independent experiments.