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. 2018 Mar 27;18:342. doi: 10.1186/s12885-018-4231-y

Fig. 2.

Fig. 2

Delphinidin induced autophagy in MDA-MB-453 cells. a Representative transmission electron microscopic images of MDA-MB-453 cells treated with DMSO (0.1%) or delphinidin (20, 40, and 80 μM) for 48 h. Arrows: autophagic vacuoles. b LC3 immunofluorescence-positive dots in MDA-MB-453 cells (× 400). c Expression of GFP-LC3-positive punctate dot (× 400). d Statistical graph of GFP-LC3-positive dots. e Expression of the LC3-II protein. f Expression of the Atg5-Atg12 conjugated protein; (g): Statistical analysis of the LC3-II/LC3-I ratio. h Statistical analysis on the ratio of Atg5-Atg12/β-actin. Dp: Delphinidin. *P < 0.05 vs control group; #P < 0.05 vs Dp group