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. 2001 Sep 18;98(20):11199–11204. doi: 10.1073/pnas.201268998

Figure 2.

Figure 2

Coimmune precipitation of Gag and Nedd-4 like protein from COS-1 cells. (A) Untransfected COS-1 cells (lanes 1 and 3) or cells transfected with pCMV-RSVGag-EGFP (lanes 2 and 4) were lysed and processed to prepare S10 extracts as described in Materials and Methods. Lanes 1 and 2 represent the input S10 extracts, whereas lanes 3 and 4 represent the S10 extracts subjected to immune precipitation with anti-Nedd4 sera. Proteins were resolved by SDS/PAGE and detected by Western blotting with an RSVGag-p19 monoclonal antibody. (B) The immunoblot described in A was reprobed by using an anti-Nedd WW domain antibody.