Figure 5.

Coimmune precipitation of RSV Gag and LDI-1 WW domains from cells. (A) Untransfected COS-1 cells (lanes 1 and 3) and cells transfected with pCMV-RSVGag-EGFP and HA-tagged LDI-1 WW domains (lanes 2 and 4) were lysed and proteins resolved and detected as described in legend to Fig. 2. Proteins in lanes 3 and 4 were immunoprecipitated with anti-HA rabbit sera before resolving the proteins by SDS/PAGE. (B) The nitrocellulose membranes described in A were reprobed in a Western analysis without stripping with a mouse anti-HA sera.