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. 2018 Mar 27;37:68. doi: 10.1186/s13046-018-0735-1

Fig. 4.

Fig. 4

Analysis of ALK fusion genes in 188 plasma samples of NSCLC patients by RT-qPCR and FISH. (a) ALK fusion genes are determined by 3′/5′ portion RT-qPCR of the ALK gene. The expression of ALK gene was calculated using 2-△△CT method. (b-c) The representative FISH images for patients with ALK fusion gene (+) (b) and ALK fusion gene (−) (c). Red arrow, split red-green dots or single red dots are indicative of ALK rearrangement; yellow arrow, or touching red-green dots are indicative of no ALK rearrangement